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- 2025年07月11日
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文献和实验The COS-7 Cell In Vitro Paradigm to Study Myelin Proteolipid Protein 1 Gene Mutations
Despite many shortcomings, a reductionist approach using cell culture paradigms to define basic principles underlying disease processes has considerable merit. One example of the utility of this approach is the expression of mutant forms
. After protein identification by mass spectrometry (MS), each peak derived from tissue protein is quantified relative to the corresponding CDIT peak. The amounts of each peak in different tissue samples can be compared relative to CDIT
Analysis of Protein Expression in Brain Tissue by ELISA
The enzyme-linked immunosorbent assay (ELISA) technique offers a sensitive, simple, and versatile method for quantifying as little as 100 pg of target protein in mixed cell or tissue lysates. A further advantage to the protocol is the ability
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