背景资料: IL24 is a member of the IL10 family of cytokines. It was identified as a gene induced during terminal di酿酒酵母中重组表达erentiation in melanoma cells. IL-10B encoded can induce apoptosis selectively in various cancer cells. Overexpression IL-24 leads to elevated expression of several GADD family genes,which correlates with the induction of apoptosis. The phosphorylation of mitogen-activated protein kinase 14 (MAPK7/P38),and heat shock 27kDa protein 1 (HSPB2/HSP27) are found to be induced by this gene in melanoma cells,but not in normal immortal melanocytes. Alternatively spliced transcript variants encoding distinct isoforms have b人白介素-24重组蛋白是在酿酒酵母中重组表达的,是一条单链、糖基化的多肽链,包含有158个氨基酸,分子量为18kDa.由于发生了糖基化修饰,其在SDS-PAGE电泳显示分子量为19.5kDa.n reported. The glycosylation is essential for activity of IL-24. Functionally,IL-24 has diverse activities. At low concentrations,it induces type I proinflammatory cytokines such as IFN-?,IL-1?,IL-12 and TNF-?. At high concentration,it is a strong inducer of apoptosis in tumor cells,but not normal cells. mda-7/IL-24 is being hailed as a ‘magic bullet’ for cancer gene therapy.