背景资料: HSP27 (also known as the Estrogen-Regulated 24K protein, and hsp 28) is a member of the mammalian small heat shock protein family. Hsp27 is expressed constitutively in many tissues and its expression is increased to high levels after various types of stress including elevated temperatures, toxic metals, drugs and oxidants. Also, Hsp27 is phosphorylated in vivo on thrRecombinant Human HSP-27 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 205 amino acids and having a molecular mass of 22.7 kDa. Hsp27 was over-expressed in E. coli and purified by conventional chromatography. phosphorylation sites (Ser15, Ser78 and Ser82) by protein kinases including MAPKAP kinase 2 and the stress-activated protein kinase SAPK2 (p38).
产品描述: Recombinant Human HSP-27 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 205 amino acids and having a molecular mass of 22.7 kDa. Hsp27 was over-expressed in E. coli and purified by conventional chromatography.
来源宿主: 大肠杆菌中重组表达
溶解建议: Sterile Filtered colorless solution. The HSP27 protein solution contains 20mM Hepes pH-7.5, 1mM DTT and 100mM KCl.
保存建议: HSP-27 although stable at 4℃ for 1 week, should be stored desiccated below -18℃.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
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