背景资料: TEV protease is the common name for the 27 kDa catalytic domain of the Nuclear Inclusion a (NIa) protein encoded by the tobacco etch virus (TEV). Because its sequence specificity is far more stringent than that of factor Xa, thrombin, or enterokinase, TEV protease is a very useful reagent for cleaving fusion proteins. TEV protease recognizes a linear epitope of the general form E-Xaa-Xaa-Y -Xaa-Q-(G/S), with cleavage occurring betwRecombinant TEV Protease (rTEV) is a site-specific protease purified from E. coli. The protease can be used for the removal of a大肠杆菌中重组表达inity tags from fusion proteins. The seven-amino-acid recognition site for rTEV is Glu-Asn-Leu-Tyr-Phe-Gln-Gly with cleavage occurring between Gln and Gly. The optimal temperature for cleavage is 30°C; however, the enzyme can be used at temperatures as low as 4°C.The rTEV contains His tag.The rTEV is purified by proprietary chromatographic techniques.n Q and G or Q and S. The most commonly used sequence is ENLYFQG.
产品描述: Recombinant TEV Protease (rTEV) is a site-specific protease purified from E. coli. The protease can be used for the removal of a大肠杆菌中重组表达inity tags from fusion proteins. The seven-amino-acid recognition site for rTEV is Glu-Asn-Leu-Tyr-Phe-Gln-Gly with cleavage occurring between Gln and Gly. The optimal temperature for cleavage is 30°C; however, the enzyme can be used at temperatures as low as 4°C.The rTEV contains His tag.The rTEV is purified by proprietary chromatographic techniques.
来源宿主: 大肠杆菌中重组表达
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溶解建议: Sterile liquid formulation. The rTEV contains 0.50 M Tris-HCl pH 8.0, 10mM DTT and 5 mM EDTA.
保存建议: Store rTEV at -70?C for long term or at -20?C for< 6 months. Please prevent freeze-thaw cycles.
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