重组人血影蛋白α

产品英文名称：
Recombinant Human Secreted Protein Acidic & Rich in Cysteine

英文简称:
SPARC

中文名称:
重组人富含半胱氨酸酸性分泌蛋白

背景资料:
SPARC， an acronym for “secreted protein， acidic and rich in cysteine”， is also known as osteonectin or BM-40. It is the founding member of a family of secreted matricellular proteins with similar domain structure. The 303 amino acid， 43 kDa protein contains a 17 aa signal sequence， an N-terminal acidic region that binds calcium， a follistatin domain containing Kazal-like sequences， and a C-terminal extracellular calcium （EC） binding domain with two EF-hand motifs. SPARC is produced by fibroblasts， capillary endothelial cells， platelets and macrophages， especially in areas of tissue morphogenesis and remodeling. SPARC shows context-specific e大肠杆菌中重组表达ects， but generally inhibits adhesion， spreading and proliferation， and promotes collagen matrix formation. For endothelial cells， SPARC disrupts focal adhesions and binds and sequesters PDGF and VEGF. SPARC is abundantly expressed in bone， where it promotes osteoblast di大肠杆菌中重组表达erentiation and inhibits adipogenesis.

产品描述:
Osteonectin Human Recombinant fused with 6X His tag produced in E.Coli is a single， non-glycosylated， polypeptide chain containing 295 amino acids and having a molecular mass of 34 kDa. The BM40 is purified by proprietary chromatographic techniques.
来源宿主:
大肠杆菌中重组表达

序列:
MSYYHHHHHHPQQEALPDETEVVEETVAEVTEVSVGANPVQVEVGEFD DGAEETEEEVVAENPCQNHHCKHGKVCELDENNTPMCVCQDPTSCP APIGEFEKVCSNDNKTFDSSCHFFATKCTLEGTKKGHKLHLDYIGPCK YIPPCLDSELTEFPLRMRDWLKNVLVTLYERDEDNNLLTKQKLRVKKI HENEKRLEAGDHPVELLARDFEKNYNMYIFPVHWQFGQLDQHPIDGY LSHTELAPLRAPLIPMEHCTTRFFETCDLDNDKYIALDEWAGCFGIKQK DIDKDLVI.

溶解建议:
Sterile Filtered White lyophilized （freeze-dried） powder. The SPARC （1 mg/ml） was lyophilized after extensive dialyses against 20mM PBS pH-7.4. It is recommended to reconstitute the lyophilized SPARC in sterile 18MΩ-cm H2O not less than 100 ug/ml， which can then be further diluted to other aqueous solutions.

保存建议：
Lyophilized Osteonectin although stable at room temperature for 3 weeks， should be stored desiccated below -18℃. Upon reconstitution BM-40 should be stored at 4℃ between 2-7 days and for future use below -18℃. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cycles.
其他简介：

  ProSpec是一家全球著名细胞因子蛋白及相关抗体生产和研发品牌。ProSpec的生产和研发工厂位于以色列，专注于蛋白（重组及合成）生产研发，其独有的细菌和哺乳动物表达和蛋白折叠技术使其能在17年内成长为国际一流的科研级蛋白供应商。目前ProSpec可以提供细胞因子，生长因子，激素，信号蛋白，病毒抗原等近800种重组蛋白和100多种抗体，是世界上提供蛋白品种最多的公司之一，领先的生产工艺和规模使其可以提供毫克到克级蛋白，价格优于同类公司。绝大部分产品是天然成熟型蛋白，而不含有标签蛋白。考虑到大部分研究者希望能更灵活地配制蛋白溶液，ProSpec-Tany绝大部分产品没有添加保护剂或盐，这就对工艺提出了更高的要求。同时，ProSpec-Tany绝大部分产品为冻干粉，因此易于运输和保存。