重组人β-痕量蛋白

产品英文名称：
Recombinant Human Phosphatase and Tensin homolog His Tag

英文简称:
PTEN His

中文名称:
重组人磷酸酶及张力蛋白同源体，His标签

背景资料:
PTEN， a tumor suppressor， has bPTEN Human Recombinant fused with 20 amino acid His tag at N-terminus produced in E.Coli is a single， non-glycosylated， polypeptide chain containing 423 amino acids （1- 403 a.a.） and having a molecular mass of 49.3kDa. The PTEN is purified by proprietary chromatographic techniques.n implicated in a large number of human tumors and is conserved from humans to worms. PTEN has a tensin like domain and a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Characterization of PTEN protein showed that it is a phosphatase that acts on proteins and on 3-phosphorylated phosphoinositides， and can therefore modulate signal transduction pathways that involve lipid second messengers. In contrast to most of the protein tyrosine phosphatases， PTEN preferentially dephosphorylates phosphoinositide substrates. PTEN negatively regulates intracellular levels of phosphatidylinositol-3，4，5-trisphosphate in cells and acts as a tumor suppressor by negative regulation of AKT/PKB signaling pathway. Recent results indicate that at least part of its role is to regulate the activity of the serine/threonine kinase AKT/PKB， and thus influence cell survival signaling.

产品描述:
PTEN Human Recombinant fused with 20 amino acid His tag at N-terminus produced in E.Coli is a single， non-glycosylated， polypeptide chain containing 423 amino acids （1- 403 a.a.） and having a molecular mass of 49.3kDa. The PTEN is purified by proprietary chromatographic techniques.
来源宿主:
大肠杆菌中重组表达

序列:
MGSSHHHHHH SSGLVPRGSH MTAIIKEIVS RNKRRYQEDG FDLDLTYIYP NIIAMGFPAE RLEGVYRNNI DDVVRFLDSK HKNHYKIYNL CAERHYDTAK FNCRVAQYPF EDHNPPQLEL IKPFCEDLDQ WLSEDDNHVA AIHCKAGKGR TGVMICAYLL HRGKFLKAQE ALDFYGEVRT RDKKGVTIPS QRRYVYYYSY LLKNHLDYRP VALLFHKMMF ETIPMFSGGT CNPQFVVCQL KVKIYSSNSG PTRREDKFMY FEFPQPLPVC GDIKVEFFHK QNKMLKKDKM FHFWVNTFFI PGPEETSEKV ENGSLCDQEI DSICSIERAD NDKEYLVLTL TKNDLDKANK DKANRYFSPN FKVKLYFTKT VEEPSNPEAS SSTSVTPDVS DNEPDHYRYS DTTDSDPENE PFDEDQHTQI TKV.

溶解建议:
Sterile Filtered colorless solution. The PTEN solution contains 20mM Tris-HCl buffer （pH 8.0）， 1mM EDTA， 2mM DTT， 100mM NaCl， and 20% glycerol.

保存建议：
PTEN although stable 4℃ for 4 weeks， should be stored desiccated below -18℃. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cycles.
其他简介：

  ProSpec是一家全球著名细胞因子蛋白及相关抗体生产和研发品牌。ProSpec的生产和研发工厂位于以色列，专注于蛋白（重组及合成）生产研发，其独有的细菌和哺乳动物表达和蛋白折叠技术使其能在17年内成长为国际一流的科研级蛋白供应商。目前ProSpec可以提供细胞因子，生长因子，激素，信号蛋白，病毒抗原等近800种重组蛋白和100多种抗体，是世界上提供蛋白品种最多的公司之一，领先的生产工艺和规模使其可以提供毫克到克级蛋白，价格优于同类公司。绝大部分产品是天然成熟型蛋白，而不含有标签蛋白。考虑到大部分研究者希望能更灵活地配制蛋白溶液，ProSpec-Tany绝大部分产品没有添加保护剂或盐，这就对工艺提出了更高的要求。同时，ProSpec-Tany绝大部分产品为冻干粉，因此易于运输和保存。