产品英文名称: Recombinant Human Tissue Transglutaminase, Sf9
英文简称: TGM2 Sf9
中文名称: 重组人组织转谷氨酰胺酶, Sf9表达
背景资料: Celiac disease is an enteropathy that is characterized by intestinal lesions of variable severity. Tissue-type transglutaminase(tTG) is believed to be the predominant autoantigen for celiac disease and the corresponding autoantibodies show higher sensitivity and specificity than anti-gliadin antibodies. Highly pure recombinant human tTG is now available to replace the traditionally used tTG fraction from guinea pig.Tissue-type transglutaminase antigens have b人组织转谷氨酰胺酶, Sf9表达重组蛋白是在杆状病毒-昆虫Sf9细胞中重组表达的,是一条糖基化的多肽链,并融合了一个6xHis标签,分子量为78,018Da.由于在活性中心进行了定点突变,其转谷酰胺酶活性被消除,同时在储存和包埋时稳定性更高.该人组织转谷氨酰胺酶, Sf9表达重组蛋白最终由标准的色谱技术纯化出.n specifically modified for improved handling: exchange of an active site amino acid eliminates the protein cross-linking activity of the enzyme, while maintaining the native thr人组织转谷氨酰胺酶, Sf9表达重组蛋白是在杆状病毒-昆虫Sf9细胞中重组表达的,是一条糖基化的多肽链,并融合了一个6xHis标签,分子量为78,018Da.由于在活性中心进行了定点突变,其转谷酰胺酶活性被消除,同时在储存和包埋时稳定性更高.该人组织转谷氨酰胺酶, Sf9表达重组蛋白最终由标准的色谱技术纯化出.-dimensional structure and the enzyme’s secondary GTPase activity. This engin人组织转谷氨酰胺酶, Sf9表达重组蛋白是在杆状病毒-昆虫Sf9细胞中重组表达的,是一条糖基化的多肽链,并融合了一个6xHis标签,分子量为78,018Da.由于在活性中心进行了定点突变,其转谷酰胺酶活性被消除,同时在储存和包埋时稳定性更高.该人组织转谷氨酰胺酶, Sf9表达重组蛋白最终由标准的色谱技术纯化出.ring assures reproducible properties of the antigen preparations through the absence of variable and ill-defined covalent aregates of tTG antigen and host cell proteins.