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文献和实验Studies of the Ubiquitin Proteasome System
and Purification of Anti‐Ubiquitin Antibodies Support Protocol 3: Preparation of Ubiquitin Affinity Column Generation of Reagent‐Grade Ubiquitin Activating Enzyme (E1) Basic Protocol 20: Preparation of Wheat E1 in E. coli Basic Protocol 21: Preparation of Mouse E1
Assays for RING Family Ubiquitin Ligases
through an enzymatic cascade of three discrete steps, which results in covalent attachment of ubiquitin to the substrate. The first two steps in this cascade involve the activating and conjugating enzymes, E1 and E2. The third and final step is performed by the E3
Selecting Patients for KIT Inhibition in Melanoma
of disease. In this chapter, we will discuss the biology of KIT in melanoma, review the rationale for and clinical data regarding KIT inhibition in melanomas harboring activating alterations of KIT , propose guidelines for the selection of patients for KIT
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