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- 2025年07月15日
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文献和实验Coimmunoprecipitation Assay for the Detection of Kinase-Substrate Interactions
Coimmunoprecipitation is a powerful tool to study protein-protein interactions and can be used to test for the physical association between a known protein kinase and its substrate. In this chapter, the author describes a protocol
In Vitro Peptidoglycan Synthesis Assay with Lipid II Substrate
proteins catalyze both of these reactions. Here, we describe an in vitro peptidoglycan synthesis assay utilizing radiolabeled lipid II substrate to monitor simultaneously peptidoglycan glycosyltransferase and transpeptidase activities. The products
Monitoring Protein Kinase A Activities Using Expressed Substrate in Live Cells
Protein kinase A (PKA) activity is regulated by intracellular cyclic adenosine mono-phosphate. Conventional protein kinase assays after cell lysis are hence not suitable for analyzing PKA activities. In this chapter, we describe a new method
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