HMGB1

产品英文名称：
Recombinant Human High-Mobility Group Box 1

英文简称:
HMGB1

中文名称:
重组人高迁移率族蛋白框1

背景资料:
High-mobility group box 1 protein （HMGB1）， previously known as HMG-1 or amphoterin， is a member of the high mobility group box family of non-histone chromosomal proteins. Human HMGB1 is expressed as a 30 kDa， 215 amino acid （aa） single chain polypeptide containing thrHMG1 Human Recombinant fused with 6X His tag produced in E.Coli is a single， non-glycosylated， polypeptide chain containing 223 amino acids and having a molecular mass of 26 kDa. The HMGB-1 is purified by proprietary chromatographic techniques. domains: two N-terminal globular， 70 aa positively charged DNA-binding domains （HMG boxes A and B）， and a negatively charged 30 aa C-terminal region that contains only Asp and Glu.4， 5 Residues 27 - 43 and 178 - 184 contain a NLS. Posttranslational modifications of the molecule have bHMG1 Human Recombinant fused with 6X His tag produced in E.Coli is a single， non-glycosylated， polypeptide chain containing 223 amino acids and having a molecular mass of 26 kDa. The HMGB-1 is purified by proprietary chromatographic techniques.n reported， with acetylation occurring on as many as 17 lysine residues. HMGB1 is expressed at high levels in almost all cells. It was originally discovered as a nuclear protein that could bend DNA. Such bending stabilizes nucleosome formation and regulates the expression of select genes upon recruitment by DNA binding proteins.

产品描述:
HMG1 Human Recombinant fused with 6X His tag produced in E.Coli is a single， non-glycosylated， polypeptide chain containing 223 amino acids and having a molecular mass of 26 kDa. The HMGB-1 is purified by proprietary chromatographic techniques.
来源宿主:
大肠杆菌中重组表达

序列:
MGKGDPKKPRGKMSSYAFFVQTCREEHKKKHPDASVNFSEFSKKCSERWKT MSAKEKGKFEDMAKADKARYEREMKTYIPPKGETKKKFKDPNAPKRPPSAFF LFCSEYRPKIKGEHPGLSIGDVAKKLGEMWNNTAADDKQPYEKKAAKLKEKY EKDIAAYRAKGKPDAAKKGVVKAEKSKKKEEEEDEEDEEDEEEEEDEEDEDE EEDDDDELEHHHHHH.

溶解建议:
Sterile Filtered White lyophilized （freeze-dried） powder. The HMG1 （1 mg/ml） was lyophilized after extensive dialyses against 1x PBS pH-7.4. It is recommended to reconstitute the lyophilized HMGB1 in sterile 18MΩ-cm H2O not less than 100 ug/ml， which can then be further diluted to other aqueous solutions.

保存建议：
Lyophilized HMGB1 although stable at room temperature for 3 weeks， should be stored desiccated below -18℃. Upon reconstitution HMGB1 should be stored at 4℃ between 2-7 days and for future use below -18℃. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cycles.
其他简介：

  ProSpec是一家全球著名细胞因子蛋白及相关抗体生产和研发品牌。ProSpec的生产和研发工厂位于以色列，专注于蛋白（重组及合成）生产研发，其独有的细菌和哺乳动物表达和蛋白折叠技术使其能在17年内成长为国际一流的科研级蛋白供应商。目前ProSpec可以提供细胞因子，生长因子，激素，信号蛋白，病毒抗原等近800种重组蛋白和100多种抗体，是世界上提供蛋白品种最多的公司之一，领先的生产工艺和规模使其可以提供毫克到克级蛋白，价格优于同类公司。绝大部分产品是天然成熟型蛋白，而不含有标签蛋白。考虑到大部分研究者希望能更灵活地配制蛋白溶液，ProSpec-Tany绝大部分产品没有添加保护剂或盐，这就对工艺提出了更高的要求。同时，ProSpec-Tany绝大部分产品为冻干粉，因此易于运输和保存。
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