背景资料: Biliverdin reductase A(BLVRA) is a member of the gfo/idh/mocA family. BLVRA is an enzyme that converts biliverdin to bilirubin, converting a double-bond betw人胆绿素还原酶A重组蛋白是在大肠杆菌中重组表达的,是一条单链、非糖基化的多肽链,包含有295个氨基酸(3-296 氨基酸和N末端Methionine),分子量为33.3kDa.该人胆绿素还原酶A重组蛋白最终由标准的色谱技术纯化出.n the second and third pyrrole ring into a single-bond. BLVRA reduces the gamma-methene bridge of the open tetrapyrrole, biliverdin IX alpha, to bilirubin with the simultaneous oxidation of a NADH or NADPH cofactor(Bilirubin + NAD(P)+ = biliverdin + NAD(P)H ).BLVRA is a regulator for induction of activating transcription factor-2 and heme oxygenase-1. Furthermore, BLVRA enhances the role of HO-1 in cytoprotection and provides cytoprotection independent of heme degradation. In addition, Bilirubin while acting as a cytoprotective antioxidant is itself oxidized to biliverdin and subsequently recycled by biliverdin reductase back to bilirubin.