ANXA11

产品英文名称：
Recombinant Human Regenerating Islet-Derived 3 Alpha

英文简称:
REG3A

中文名称:
重组人胰腺再生蛋白3α

背景资料:
Pancreatitis-associated protein （PAP） is a secretory protein not normally expressed in healthy pancreas but highly induced during acute pancreatitis. While PAP has bThe Recombinant Human REG3A protein is produced with N-terminal fusion of His Tag. The REG3A His -Taed Fusion Protein is an 18.4 kDa protein containing 149 amino acid residues of the Human REG3A and 16 additional amino acid residues- His Tag （underlined）. MRGSHHHHHH GMASHMEEPQ RELPSARIRC PKGSKAYGSH CYALFLSPKS WTDADLACQK RPSGNLVSVL SGAEGSFVSS LVKSIGNSYS YVWIGLHDPT QGTEPNGEGW EWSSSDVMNY FAWERNPSTI SSPGHCASLS RSTAFLRWKD YNCNVRLPYV CKFTD.n shown to be anti-bacterial and antiapoptotic in vitro， its definitive biological function in vivo is not clear. Using antisepse oligonucleotides， inhibition of PAP expression significantly worsened pancreatitis in a rat model. During pancreatitis， PAP released by the pancreas could mediate lung inflammation through induction of hepatic TNF- alpha expression and subsequent increase in circulating TNF-alpha. PAP is activated in primary liver cancers. In normal liver， the protein is undetectable in normal mature hepatocytes and found only in some ductular cells， representing potential hepatic progenitor cells. PAP can be considered hepatic cytokine that combines mitogenic and anti-apoptotic functions regarding hepatocytes， and consequently acts as a growth factor in vivo to enhance liver regeneration. In pancreatic cancor， PAP was overexpressed in 79% （30 of 38） of pancreatic ductal adenocarcinoma， 19% （7 of 36） of chronic pancreatitis， and 29% （2 of 7） of mucinous cystadenoma. PAP was found in malignant ductular structures in pancreatic carcinomas as well as in benign proliferating ductules and acinar cells in chronic pancreatitis. Elevation of PAP in patients with pancreatic cancer is not merely explainable by concomitant pancreatitis， but sThe Recombinant Human REG3A protein is produced with N-terminal fusion of His Tag. The REG3A His -Taed Fusion Protein is an 18.4 kDa protein containing 149 amino acid residues of the Human REG3A and 16 additional amino acid residues- His Tag （underlined）. MRGSHHHHHH GMASHMEEPQ RELPSARIRC PKGSKAYGSH CYALFLSPKS WTDADLACQK RPSGNLVSVL SGAEGSFVSS LVKSIGNSYS YVWIGLHDPT QGTEPNGEGW EWSSSDVMNY FAWERNPSTI SSPGHCASLS RSTAFLRWKD YNCNVRLPYV CKFTD.ms to be due to increased PAP production by the cancer cells and is also correlated to tumour load as expressed by the UICC stages. Epithelial expression of PAP was induced under intestinal mucosal inflammation initiated by exposure to commensal bacteria or DSS as well as inflamed IBD colon. Increased serum level of PAP diagnosed ileal location in active Crohn disease with a sensitivity of 60%， a specificity of 94%， a positive predictive value of 84% and a negative predictive value of 81%. Elevated serum PAP （> 50 ng/mL） is significantly associated with disease activity and ileal location of Crohn disease.

产品描述:
The Recombinant Human REG3A protein is produced with N-terminal fusion of His Tag. The REG3A His -Taed Fusion Protein is an 18.4 kDa protein containing 149 amino acid residues of the Human REG3A and 16 additional amino acid residues- His Tag （underlined）. MRGSHHHHHH GMASHMEEPQ RELPSARIRC PKGSKAYGSH CYALFLSPKS WTDADLACQK RPSGNLVSVL SGAEGSFVSS LVKSIGNSYS YVWIGLHDPT QGTEPNGEGW EWSSSDVMNY FAWERNPSTI SSPGHCASLS RSTAFLRWKD YNCNVRLPYV CKFTD.
来源宿主:
大肠杆菌中重组表达

序列:


溶解建议:
Filtered White lyophilized （freeze-dried） powder. Filtered （0.4um） and lyophilized from 0.5 mg/ml in 0.05M Acetate buffer pH-4. It is recommended to add 0.1M Acetate buffer pH-4 to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. For conversion into higher pH value， we recommend intensive dilution by relevant buffer to a concentration of 10ug/ml. In higher concentrations the solubility of this antigen is limited. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

保存建议：
Store lyophilized protein at -20℃. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4℃ for a limited period of time； it does not show any change after two weeks at 4℃. The lyophilized protein remains stable until the expiry date when stored at -20℃.
其他简介：

  ProSpec是一家全球著名细胞因子蛋白及相关抗体生产和研发品牌。ProSpec的生产和研发工厂位于以色列，专注于蛋白（重组及合成）生产研发，其独有的细菌和哺乳动物表达和蛋白折叠技术使其能在17年内成长为国际的科研级蛋白供应商。目前ProSpec可以提供细胞因子，生长因子，激素，信号蛋白，病毒抗原等近800种重组蛋白和100多种抗体，是世界上提供蛋白品种最多的公司之一，领先的生产工艺和规模使其可以提供毫克到克级蛋白，价格优于同类公司。绝大部分产品是天然成熟型蛋白，而不含有标签蛋白。考虑到大部分研究者希望能更灵活地配制蛋白溶液，ProSpec-Tany绝大部分产品没有添加保护剂或盐，这就对工艺提出了更高的要求。同时，ProSpec-Tany绝大部分产品为冻干粉，因此易于运输和保存。