背景资料: Endonuclease III(nth) is a DNA repair enzyme which has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases numerous damaged pyrimidines from DNA by cleaving the N-glycosidic bond and leaving an AP(apurinic/apyrimidinic) site. This AP-lyase activity cleaves the phosphodiester bond 3’ to the AP site by a beta-elimination, thus leaving a 3’-terminal unsaturated sugar and a product with a terminal 5’-phosphate.
产品描述: NTH E.Coli Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 231 amino acids(1-211a.a.) and having a molecular mass of 25.7kDa. The NTH is purified by proprietary chromatographic techniques.
来源宿主: 大肠杆菌中重组表达