背景资料: Interleukin-22 (IL-22),also known as IL-10-related T cell-derived inducible factor (IL-TIF) was initially identified as a gene induced by IL-9 in mouse T cells and mast cells. Mouse IL-22 cDNA encodes a 179 amino acid residue protein with a putative 33 amino acids signal peptide that is cleaved to generate a 147 aa mature protein that shares approximately 79% and 22% aa sequence identity with human IL-22 and IL-10,respectively. IL-22 has b小鼠白介素-22,PEG修饰重组蛋白是在大肠杆菌中重组表达的,是两条非糖基化多肽链组成的同源二聚体,每条多肽链包含有147个氨基酸,在其N末端添加了一个额外的Ala氨基酸,其分子量为36kDa.然而,由于发生了糖基化修饰而发生的水动力学效应,其在SDS-PAGE电泳中的显示分子量为50 kDa,而在Superdex 200的凝胶过滤中显示分子量为200 kDa.该单PEG修饰的小鼠白介素-22重组蛋白最终由标准的色谱技术纯化出.n shown to activate STAT-1 and STAT-3 in several hepatoma cell lines and upregulate the production of acute phase proteins. IL-22 is produced by normal mouse T cells upon Con A activation. Mouse IL-22 expression is also induced in various organs upon lipopolysaccharide injection,suesting that IL-22 may be involved in inflammatory responses. The functional IL-22 receptor complex consists of two receptor subunits,IL-22R (previously an orphan receptor named CRF2-9) and IL-10Rβ (previously known as CRF2-4),belonging to the class II cytokine recep