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- Cytoskeleton
- 美国
- CS-GE04
- 2025年07月09日
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参照说明书
- 英文名：
Tiam1 GEF protein (human recomb. DHPH domain, MBP tagged)
- 库存：
1
- 供应商：
北京博蕾德生物科技有限公司
- 规格：
1x100ug
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文献和实验HIS-tagged protein purification
1) grow 20ml cells O/N 37 C, dilute 50X into prewarmed LB, grow to 0.6 OD or about 1hr. Induce w/ 2mM IPTG (238mg/0.5l), grow 3hr, spin 6k GS3 10', freeze at -70 ℃.2) resuspend cells (from 500ml) in fliptop in 25 mls. UPB8.0, freeze lN2.3) thaw
HIS-tagged protein purification
John Mundy,Institute of Molecular Biology,Copenhagen,Denmark 1 liter cell prep 1)grow 20ml cells O/N37℃ dilute 50X into prewarmed LB,grow to 0.6 OD or about 1hr.Induce w/ 2mM IPTG (238mg/0.5l),grow 3hr,spin 6k GS3 10',freeze at 70℃ 2)resuspend
Proteins are composed of functional units, or domains, that can be found alone or in combination with other domains. Analysis of protein domain architectures and the movement of protein domains within and across different genomes provide
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