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- AbD Serotec
- STAR91
- 2025年08月21日
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文献和实验Anti-GFP/BrdU In Situ Immunostaining
IgG (Jackson ImmunoResearch, 111-096-006) Biotinylated anti-BrdU (AbCam, ab2284-125) Streptavidin-Alexa Flour 555 (Invitrogen/Molecular Probes, S21381) Goat serum Horse serum BSA 2N HCl DAPI Fluorescent mounting medium (Vectashield
, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
Immunohistochemistry: Fluorescence Protocol 2.0
Immuno Research Lab Catalog # 705-066-147) Biotinylated goat anti-mouse IgG1 ( Caltag Lab Catalog # M32115) FITC-labeled goat anti-mouse IgG1 ( Caltag Lab Catalog # M32001) FITC-labeled anti-mouse IgG2A ( Caltag Lab Catalog # M
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