产品信息:
Several lines of evidence highlight the significance of HMGB1 in the immune inflammatory response. For example, it has been shown that HMGB1 is actively released by a variety of cells such as macrophages when stimulated by lipopolysaccharides (LPS), TNF-a, and IL-1 , and is passively released by injured or necrotic cells associated with collapsing cell structures. In fact, patients who died from septic shock had higher serum HMGB1 levels than surviving sepsis patients. Similarly, high serum HMGB1 levels are observed in sepsis animal models and in collagen-induced arthritis animal models. With regard to the function of the protein itself, HMGB1 has also been shown to stimulate the release of TNF-a and IL-1, as well as bind LPS and synergistically increase peripheral blood mononuclear cell IL-6 production. Together, these observations demonstrate that HMGB1 plays important roles in the inflammatory cascade.
Chondrex, Inc. provides a capture ELISA kit to determine HMGB1 levels in cell culture media and sera. This kit contains enough reagents to measure 40 samples in duplicate together with standards.