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Human IFN-gamma/IL-2/TNF-alpha

FluoroSpot kit, pre-coated
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  • 艾美捷代理品牌
  • FSP-010209-10
  • 2025年07月13日
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    • 文献和实验
    • 技术资料
    • 英文名

      Human IFN-gamma/IL-2/TNF-alpha FluoroSpot kit, pre-coated

    • 供应商

      艾美捷科技

    • 规格

      10plates

    产品名称:Human IFN-gamma/IL-2/TNF-alpha FluoroSpot kit, pre-coated-Human IFN-gamma/IL-2/TNF-alpha FluoroSpot kit, pre-coated

    产品货号:FSP-010209-10

    产品规格:10plates

    背景资料:Interferon-γ (IFN-γ, also known as IFN-gamma, IFN-g, IFNg and Interferon-gamma) is produced mainly by activated T cells and NK cells. It is a proinflammatory cytokine that activates macrophages and endothelial cells, but it also regulates immune responses by effecting APC and T and B cells. Production of IFN-γ by helper T cells as well as cytotoxic T cells is a hallmark of the TH1-type phenotype, thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens./Interleukin-2 (IL-2, IL2) is produced exclusively by activated T cells. IL-2 promotes the proliferation, differentiation, and survival of antigen-activated T cells and NK-cells./Tumor necrosis factor-α (TNF-α, also known as TNF-alpha, TNF-a, TNFa, and Tumor necrosis factor-alpha) is produced by many different cell types e.g. monocytes, macrophages, T cells and B cells. Among the many effects of TNF-α, are protection against bacterial infection, cell growth modulation, immune system regulation and involvement in septic shock.

    Mabtech代理艾美捷科技

    保存建议:Human IFN-gamma/IL-2/TNF-alpha FluoroSpot kit, pre-coated-Human IFN-gamma/IL-2/TNF-alpha FluoroSpot kit, pre-coated建议收到产品后2-8°C保存,有效期为12个月。

    产品描述:The FluoroSpot assay is an inventive development of the ELISpot assay and uses fluorescent detection instead of colorimetric. This allows for simultaneous detection of several analytes, which is a major advantage. The human IFN-γ/IL-2/ TNF-α FluoroSpot kit is perfect for users who wish to have a convenient assay with minimized assay variability. It enables enumeration of cells secreting one, two or three analytes. The kit includes low fluorescent plates pre-coated with monoclonal capture antibodies, monoclonal detection antibodies, secondary reagents conjugated to fluorophores, a fluorescence enhancer and an anti-CD28 monoclonal antibody for co-stimulation. A polyclonal activator for human T cells (mAb CD3-2) is supplied as a positive control for cytokine secretion.

    点击:Human IFN-gamma/IL-2/TNF-alpha FluoroSpot kit, pre-coated-Human IFN-gamma/IL-2/TNF-alpha FluoroSpot kit, pre-coated查看更详细产品说明,更多应用、储存、价格、货期等信息请致电垂询Mabtech中国区域授权总代理艾美捷科技有限公司。更多特色试剂盒、特色抗体以及特色试剂等产品评论,请点击查看每日生物评论网站。

    Mabtech代理艾美捷科技

    Mabtech 公司是一家欧洲的集研究,开发,销售,技术支持为一体的高科技免疫生物技术公司。Mabtech公司始终专业致力于研发新的单克隆抗体,生产创新的免疫试剂和销售高品质的全配套的免疫试剂盒。公司核心业务是致力于优化发展酶联免疫斑点法技术 (ELISpot)、荧光免疫斑点法技术 (FluoroSpot) 和酶联免疫吸附试验技术 (ELISA) 等免疫学方法。作为Mabtech在中国的区域代理,艾美捷科技有限公司将为中国客户提供全面的Mabtech产品以及客户订制化服务。

    艾美捷科技有限公司品线覆盖生物研究各个领域,主要包括:分子生物学(核酸蛋白研究工具、cDNA/RNA、转染试剂、植物组培/细胞培养、生化试剂及试剂盒),试剂(蛋白酶抑制剂、化合物、荧光染料/探针、动物血清)、抗体(一抗、二抗、封闭血清), 蛋白及试剂盒(细胞因子/重组蛋白、细胞凋亡/代谢/损伤、表观遗传、天然免疫)等。艾美捷为您寻找并提供最优的产品解决方案、最完备的物流服务、最及时的售后服务。如果您对上述产品线感兴趣,请致电免费服务热线到艾美捷垂询相关产品信息及完整实验解决方案。

    关键词:细胞分析试剂盒,Human IFN-gamma/IL-2/TNF-alpha FluoroSpot kit, pre-coated, IFN-gamma/IL-2/TNF-alpha FluoroSpot kit

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    图标文献和实验
    相关实验
    • Dual- and Triple-Color Fluorospot

      3, and Cy5. 3. Fluorescence Enhancer (Mabtech) is used to improve the fluorescent signal. 实验步骤 The protocol describes double staining for the detection of human IFN-γ and IL-2. PVDF plates are coated with mAbs to IFN-γ and IL-2

    • Human DC Enrichment Kit

      volume of 35 ml at room temperature. 2)        Add the diluted buffy coat on top of 15 ml of Lymphoprep. 3)        Centrifuge at 160 x g for 20 minutes at 20°C. Allow to decellerate without brakes. 4)        Remove 20 ml of supernatant

    • Reprogramming Fibroblasts with the CytoTune-iPS Reprogramming Kit

      9. GlutaMAX™-I Supplement 10. Basic FGF, Recombinant Human 11. β-Mercaptoethanol, 1000X 12. Penicillin-Streptomycin, Liquid 13. Attachment Factor 14. TrypLE™ Select Cell Dissociation Reagent or 0.05% Trypsin/EDTA

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