Product overview
Description Anti-HLA-B rabbit polyclonal antibody
Applications ELISA, WB
Immunogen Synthetic peptide of human HLA-B
Reactivity Human
Content 0.36 mg/ml
Host species Rabbit
Ig class Immunogen-specific rabbit IgG
Purification Antigen affinity purification
Target information
Symbol HLA-B
Full name major histocompatibility complex, class I, B
Synonyms AS; HLAB; B-4901
Swissprot P01889
Target Background
HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exon 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described.
Anti-HLA-B antibody Applications
Western blotting
Predicted band size:40 kDa
Positive control:A549 cell lysate
Recommended dilution: 200-1000