Matrices listed below were spiked with certain level of EPHA1 and the recovery rates were calculated by comparing the measured value to the expected amount of EPHA1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-103
96
EDTA plasma(n=5)
87-101
92
heparin plasma(n=5)
88-105
99
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of EPHA1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.