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文献和实验全基因组的比较基因组杂交技术介绍(Whole-Genome and Custom Fine-Tiling Array CGH)
aberrations identified in whole-genome surveys can be readily examined at even higher resolution with custom fine-tiling array CGH. Such studies can map genomic changes down to the gene and even exon level and enable breakpoint mapping to less than 500 bp
在RT-PCR中避免DNA污染(Avoiding DNA Contamination in R
present in total RNA samples isolated by most commonly used techniques. To illustrate this problem, we performed RT-PCR on mouse liver RNA isolated by a multi-step guanidinium thiocyanate/acid phenol:chloroform extraction (ToTALLY RNA™), a one-step
the collection tubes for next step. 6) Add 700 ul of SPW Wash buffer diluted with ethanol to the HiBind™ MicroElute™ DNA column and spin at 10,000 x g for 1 minute. Discard the flow-through and place the column back into same 2mL collection tube.
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