Matrices listed below were spiked with certain level of GATA4 and the recovery rates were calculated by comparing the measured value to the expected amount of GATA4 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-105
95
EDTA plasma(n=5)
86-105
99
heparin plasma(n=5)
86-100
93
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GATA4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.