Matrices listed below were spiked with certain level of NF-κB p65 and the recovery rates were calculated by comparing the measured value to the expected amount of NF-κB p65 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-99
95
EDTA plasma(n=5)
89-93
90
heparin plasma(n=5)
86-99
91
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NF-κB p65 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.