LS-F10298 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human Human Lambda Light Chain in samples of Plasma and Serum. It is based upon a Competitive EIA assay principle and can be used to detect levels of Human Lambda Light Chain as low as 0.021 micrograms per millilter.
Specifications
Type
Competitive EIA (enzyme immunoassay) kit
Target
Human Lambda Light Chain
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of human ?-IgLC. No significant cross-reactivity or interference between human ?-IgLC and analogs was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between human ?-IgLC and all the analogs, therefore, cross reaction may still exist.
Manual
Intended Sample Types
Plasma, Serum
Sample Size
50 - 100 µl
Format
96-Well Microplate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
0.039 - 2.5 µg/ml
Sensitivity
0.021 µg/ml
Precision
Intra-assay: CV%<8% Inter-assay: CV%<10%
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
User Manual
96-well Microplate
Detection Reagents
Wash Buffer
Substrate
Stop Solution
Adhesive Plate Sealers
Background
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with ?-IgLC. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for ?-IgLC. The competitive inhibition reaction is launched between with pre-coated ?-IgLC and ?-IgLC in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of ?-IgLC in the samples. The color development is stopped and the intensity of the color is measured.
Restrictions