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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
常温
- 保质期:
见标签
- 库存:
现货
- 供应商:
联硕
- CAS号:
73590-58-6
- 规格:
100mg
一般描述
应用
生化/生理作用

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文献和实验Replication timing by comparative hybridization
the culture to the 37o bath. 5. Once the culture has reached 37o C (it takes 10-15 min), add Pronase (0.01-0.05 mg/ml final conc) to release the cells from the alpha factor block. [You can add dry Pronase. I usually dissolve the Pronase in 5 ml
Rinse in distilled water for 5 min Stain in carmine alum O/N Wash in 70 % EtOH 15 min Wash in 95 % EtOH 15 min Wash in 100 % EtOH 15 min Clear in xylene and mount with Permount After photographic documentation the tissue
Replication timing by density transfer
. (I add 0.1 mg/ml ampicillin if I'm feeling paranoid.) The doubling time of RM14-3a is ~ 2.6 hr at 23o C in glucose and 6-8 hr (depending on aeration) in acetate--longer if you're selecting for a plasmid. RM14-3a is MATa, cdc7-1ts , bar1, trp1-289
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