For use in permeabilization、 dilute Permeabilization Wash Buffer (10X) to 1X in DI water. Resuspend fixed cells in diluted Permeabilization Wash Buffer and centrifuge at 350 xg for 5-10 minutes、 and repeat the same process for twice. It is recommended that the reagent be titrated for optimal performance for each application. Please see Intracellular Cytokine Staining Protocol" under "Support" on BioLegend's website.
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Storage
This permeabilization wash buffer solution should be stored at 4°C. Do not freeze.