Ba/F3 cell line transfected with mouse TLR4 /MD2 complex
Formulation
0.2 µm filtered in phosphate-buffered solution、 pH 7.2、 containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin、 Azide-Free) antibody was purified by affinity chromatography.
Concentration
1mg/ml
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining、 the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Storage
The antibody solution should be stored undiluted at 4°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.