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文献和实验Expression and Isolation of Recombinant Antibody Fragments in E. coli
linker into expression constructs to produce scFv. Further variants include the addition of a range of suitable (C-terminal) tags for the purification or detection of expressed protein, such as a human Cκ domain (scAb) (2 ), c-myc, or hexahistidine.
Symplex™ is an antibody discovery technology that identifies fully human antigen-specific antibody repertoires directly from plasma cells. The technology utilizes reverse transcription and overlap extension polymerase chain reaction performed
Expression of Recombinant Cytochromes c in E. coli
for proper folding and stability. However, significant advances in expression of recombinant cytochromes c have been made during the last decade. It has been shown that a single gene cluster, ccmA–H , is responsible for cytochrome c maturation in Escherichia
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