Anti-IST1 antibody

Anti-IST1 antibody

Cat. No.     JC264281
Package    25 μl/100 μl/200 μl
Storage    -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Product overview
Description                   Anti-IST1 rabbit polyclonal antibody
Applications        ELISA, IHC 
Immunogen               Synthetic peptide of human IST1
Reactivity                 Human, Mouse, Rat
Content                     1.08 mg/ml
Host species               Rabbit
Ig class                  Immunogen-specific rabbit IgG
Purification                 Antigen affinity purification

Target information
Symbol                 IST1
Full name                 IST1, ESCRT-III associated factor
Synonyms    OLC1
Swissprot    P53990

Target Background
This gene encodes a protein with MIT-interacting motifs that interacts with components of endosomal sorting complexes required for transport (ESCRT). ESCRT functions in vesicle budding, such as that which occurs during membrane abscission in cytokinesis. There is a pseudogene for this gene on chromosome 19. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Applications
Immunohistochemistry
Predicted cell location: Cytoplasm and Nucleus
Positive control: Human colorectal cancer
Recommended dilution: 40-200    Predicted cell location: Cytoplasm and Nucleus
Positive control: Human tonsil
Recommended dilution: 40-200
            
The image on the left is immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using ml264281(IST1 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200)    The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using ml264281(IST1 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200)

ELISA
Recommended dilution: 5000-10000