Human HIF1A / HIF1 Alpha ELISA

Kit (Sandwich ELISA) - LS-F33088

价格$865

品牌LSBio
检测范围0.156 - 10 ng/ml
货号LS-F33088
产地美国
更新日期2025年07月02日

11 年钻石会员

Product Description

LS-F33088 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human HIF1A / HIF1 Alpha in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of HIF1A / HIF1 Alpha as low as 0.094 nanograms per millilter.

Specifications

Type

Sandwich ELISA (enzyme-linked immunosorbent assay) kit
Target

HIF1A / HIF1 Alpha
Reactivity

Human
Manual

Intended Sample Types

Plasma, Serum, Tissue Homogenates
Format

96-Well Strip Plate
Detection

Colorimetric - 450nm (TMB)
Measurement

Quantitative
Detection Range

0.156 - 10 ng/ml
Sensitivity

0.094 ng/ml
Precision

Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage

Store at 4°C. Stable for 6 months.
Quality Assurance

Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components

User Manual
96-well Microplate
Detection Reagents
Wash Buffer
Substrate
Stop Solution
Adhesive Plate Sealers

Background

This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- HIF1Aantibody was pre-coated onto 96-well plates. And the biotin conjugated anti- HIF1A antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP- Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the HIF1A amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of HIF1A can be calculated.
Restrictions

For research use only.
Guarantee

This elisa kit carries the LSBio 100% Guarantee.
About HIF1A / HIF1 Alpha

Uniprot: Q16665 NCBI: NM_001530 NP_001521.1