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- 详细信息
- 文献和实验
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- 库存:
99
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已收到实际货物为准
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北京诺博莱德科技有限公司
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已收到实际货物为准
详细信息:
| 克隆号 : | BC28 |
| Ig分类 : | IgG1 |
| 适用组织: | 石蜡切片 |
| 阳性部位: | 细胞核 |
| 阳性对照: | 肺鳞癌 |
| 特异性 : | 此抗体与人来源的p40蛋白反应,其它种属未检测。 |
| 推荐效价: | •原液使用前应以适当比例稀释 推荐稀释度为1:50~1:100 •工作液不必稀释,直接滴加使用 |
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文献和实验Monoclonal Antibody Production
Mouse Immunization Order 6 six week old Balb/C mice and let the ARC know they are coming. Have your antigen ready for when they arrive. Once they get there earmark the mice and perform a pre-bleed on them to be used as an ELISA control
Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
Protocol for anti-HA antibody Western Blotting
seconds, and pour it off: this method was worked out in the Yamamoto lab eons ago). 7) Incubate with secondary antibody (anti-mouse) in 5% BSA/PBS/Tween as you would ordinarily. I usually go for 2 hours @ room temp. 8) 12-15 quick washes again(in 1XPBS
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