- $800
- LSBio
- To be determined
- LS-F10270
- 美国
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 样本：
血清/血浆/组织匀浆液
- 库存：
1000
- 适应物种：
Human
- 应用：
科研使用
- 检测方法：
ELISA
- 检测范围：
To be determined
- 供应商：
康朗生物
- 规格：
96T
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Product Description
LS-F10270 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Qualitative detection of Human HDV / Hepatitis D Virus in samples of Serum. It is based upon a Indirect assay principle.Specifications
TypeELISA (enzyme-linked immunosorbent assay) kit
Target
HDV / Hepatitis D Virus
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of human HDV antibody(IgM). No significant cross-reactivity or interference between human HDV antibody(IgM) and analogs was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between human HDV antibody(IgM) and all the analogs, therefore, cross reaction may still exist.
Manual
Intended Sample Types
Serum
Sample Size
50 - 100 µl
Format
96-Well Microplate
Detection
Colorimetric - 450nm (TMB)
Measurement
Qualitative
Precision
Intra-assay: CV%<15% Inter-assay: CV%<15%
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
- Coated assay plate
- Negative Control
- Positive Control
- Sample Diluent
- HRP-conjugate
- 2 x Wash Buffer (30 x concentrate)
- Substrate A
- Substrate B
- Stop Solution
- Adhesive Strip (For 96 wells)
Background
This assay employs the qualitative enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with antigen. Samples are pipetted into the wells with anti-human IgM conjugated Horseradish Peroxidase (HRP). Any antibodies specific for the antigen present will bind to the pre-coated antigen. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of human HDV antibody(IgM) bound in the initial step. The color development is stopped and the intensity of the color is measured.
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.
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Range: Positive/Negative
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Direct ELISA Platform Overview
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文献和实验Reprogramming Fibroblasts with the CytoTune-iPS Reprogramming Kit
9. GlutaMAX™-I Supplement 10. Basic FGF, Recombinant Human 11. β-Mercaptoethanol, 1000X 12. Penicillin-Streptomycin, Liquid 13. Attachment Factor 14. TrypLE™ Select Cell Dissociation Reagent or 0.05% Trypsin/EDTA
Bianchi, E., Steinkuhler, C., Taliani, M., Urbani, A., Francesco, R.D., and Pessi, A. 1996. Synthetic depsipeptide substrates for the assay of human hepatitis C virus protease. Anal. Biochem. 237:239‐244
有替代前者的趋势。由于ABS-ELISA较普通ELISA多用了两种试剂，增加了操作步骤，在临床检验中ABS-ELISA应用不多。科研项目中检测微量的成分如细胞因子常采用本法。晶美分装ELISA KIT采用的方法：1， TORCH及传染病试剂盒（间接法），见2.2.32， TORCH-IgM捕获法特色：包被抗体，标记抗原原理：3， 细胞因子试剂盒采用的方法路线（ABC-ELISA）原理产品特色：采用ABC法，灵敏度更高，特异性更强。生物素抗体和酶联物是浓缩的，使用前需用相应的缓冲液稀释。酶联物可以通用
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