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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海联迈生物工程有限公司
- 库存:
大量
- 靶点:
详见说明书
- 级别:
1
- 目录编号:
LM-10395R-FITC
- 克隆性:
多克隆
- 抗原来源:
Rabbit
- 保质期:
1年
- 抗体英文名:
Anti-TIMP-2/FITC
- 抗体名:
Anti-TIMP-2/FITC
- 标记物:
FITC标记
- 宿主:
Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep,
- 适应物种:
Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep,
- 免疫原:
详见说明书
- 亚型:
IGg
- 形态:
粉末、液体、冻干粉
- 应用范围:
ICC=1:50-200 IF=1:50-200
- 浓度:
1mg/ml
- 保存条件:
-20 °C
- 规格:
100ul
| 英文名称 | Anti-TIMP-2/FITC |
| 中文名称 | FITC标记的金属蛋白酶组织抑制因子-2抗体 |
| 别 名 | Metalloproteinase inhibitor 2 precursor; Tissue inhibitor of metalloproteinases 2; Collagenase inhibitor; CSC 21K; CSC21K; TIMP2; TIMP 2; TIMP 2; TIMP metallopeptidase inhibitor 2; TIMP2_HUMAN; Metalloproteinase inhibitor 2; CSC-21K; TIMP-2. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep, |
| 产品应用 | ICC=1:50-200 IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 24kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TIMP-2 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: This gene is a member of the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. In addition to an inhibitory role against metalloproteinases, the encoded protein has a unique role among TIMP family members in its ability to directly suppress the proliferation of endothelial cells. As a result, the encoded protein may be critical to the maintenance of tissue homeostasis by suppressing the proliferation of quiescent tissues in response to angiogenic factors, and by inhibiting protease activity in tissues undergoing remodelling of the extracellular matrix. [provided by RefSeq, Jul 2008]. Function: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19. Subunit: Interacts (via the C-terminal) with MMP2 (via the C-terminal PEX domain); the interaction inhibits the MMP2 activity. Subcellular Location: Secreted. Post-translational modifications: The activity of TIMP2 is dependent on the presence of disulfide bonds. Similarity: Belongs to the protease inhibitor I35 (TIMP) family. Contains 1 NTR domain. Database links: Entrez Gene: 282093 Cow Entrez Gene: 100135629 Guinea pig Entrez Gene: 7077 Human Entrez Gene: 21858 Mouse Entrez Gene: 100008689 Rabbit Entrez Gene: 29543 Rat Omim: 188825 Human SwissProt: P16368 Cow SwissProt: Q9WUC6 Guinea pig SwissProt: P16035 Human SwissProt: P25785 Mouse SwissProt: Q9TRZ7 Rabbit SwissProt: P30121 Rat Unigene: 633514 Human Unigene: 206505 Mouse Unigene: 10161 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
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文献和实验人金属蛋白酶组织抑制因子-2(TIMP-2)酶联免疫分析(ELISA)
板,制成固相抗体,往包被单抗的微孔中依次加入 人 金属蛋白酶组织抑制因子 -2(TIMP-2) , 再与 HRP 标记的金属蛋白酶组织抑制因子 -2(TIMP-2) 抗体结合,形成抗体 - 抗原 - 酶标抗体复合物,经过彻底洗涤后加底物 TMB 显色。 TMB 在 HRP 酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的 人 金属蛋白酶组织抑制因子 -2(TIMP-2) 呈正相关。用酶标仪在 450nm 波长下测定吸光度( OD 值),通过标准曲线计算样品中 人 金属蛋白酶
人金属蛋白酶组织抑制因子-9(TIMP-9)酶联免疫分析(ELISA)
包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入 TIMP-9 , 再与 HRP 标记的 TIMP-9 抗体结合,形成抗体 - 抗原 - 酶标抗体复合物,经过彻底洗涤后加底物 TMB 显色。 TMB 在 HRP 酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的金属蛋白酶组织抑制因子 -9 (TIMP-9) 呈正相关。用酶标仪在 450nm 波长下测定吸光度( OD 值),通过标准曲线计算样品中人金属蛋白酶组织抑制因子 -9 (TIMP-9) 浓度。 试剂
人金属蛋白酶组织抑制因子-1(TIMP-1)酶联免疫分析(ELISA)
,往包被单抗的微孔中依次加入 人 金属蛋白酶组织抑制因子 -1(TIMP-1) , 再与 HRP 标记的金属蛋白酶组织抑制因子 -1(TIMP-1) 抗体结合,形成抗体 - 抗原 - 酶标抗体复合物,经过彻底洗涤后加底物 TMB 显色。 TMB 在 HRP 酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的 人 金属蛋白酶组织抑制因子 -1(TIMP-1) 呈正相关。用酶标仪在 450nm 波长下测定吸光度( OD 值),通过标准曲线计算样品中 人 金属蛋白酶组织抑制因子
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