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- Sigma
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- T4009-250MG
- 2026年01月20日
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常温
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- 供应商：
联硕生物
- CAS号：
345909-26-4
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1瓶
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文献和实验Native Chromatin Preparation and Illumina/Solexa Library Construction
Protease inhibitor cocktail for ChIP (1000X) Proteinase K (20 mg/mL) (Roche Diagnostics) Reagents for agarose gel electrophoresis RIPA buffer for ChIP RNase A SDS (sodium dodecyl sulfate) (10%) T cell isolation buffer
above, but following DNA precipitation by polyethylene glycol, the DNA pellet is resuspended in RNase buffer and treated with RNase A and T1. Nuclease treatment is necessary to remove the RNA by digestion since RNA competes with the DNA for binding to the diatomaceous
glycogen (20 mg/ml), 21 µl 4M ammonium acetate, 90 µl EtOH and ppt. 4. Resuspend pellet in 26 µl water, and add 26 µl 4M ammonium acetate, 110 µl EtOH and ppt. again. 5. Resuspend pellet in 51 µl water. PCR-generated single-stranded DNA probe 20 µl
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