Rabbit polyclonal antibody against 14-3-3 zeta protein. Immunogen region is Internal. Specificity is as follows for the reactive species: H:S58, M:S58, R:S58.
Target
14-3-3 zeta
Reactivity
Human, Mouse, Rat
Host
Rabbit
Clonality
Polyclonal
Tested Applications
ELISA, WB, IHC, IF/ICC
Recommended dilutions
WB: 1/500 - 3000, IHC: 1/50 - 100, IF/ICC: 1/100 - 1/500, ELISA: 1/40000. Optimal dilutions/concentrations should be determined by the end user.
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human 14-3-3 zeta around the phosphorylation site of serine 58 (R-S-SP-W-R).
Purification
Purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen.
Form
Liquid
Isotype
IgG
Conjugation
Unconjugated
Storage
Aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles.
Swiss Prot
P63104
Buffer
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% sodium azide, 50% glycerol.