Product overview
Description Anti-UPF3A rabbit polyclonal antibody
Applications ELISA, IHC
Immunogen Synthetic peptide of human UPF3A
Reactivity Human
Content 0.4 mg/ml
Host species Rabbit
Ig class Immunogen-specific rabbit IgG
Purification Antigen affinity purification
Target information
Symbol UPF3A
Full name UPF3 regulator of nonsense transcripts homolog A (yeast)
Synonyms UPF3; HUPF3A; RENT3A
Swissprot Q9H1J1
Target Background
This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. The encoded protein is one of two functional homologs to yeast Upf3p. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctions. This gene is located on the long arm of chromosome 13. Two splice variants encoding different isoforms have been found for this gene.
Applications
Immunohistochemistry
Predicted cell location: Cytoplasm or Nucleus
Positive control: Human brain
Recommended dilution: 20-100 Predicted cell location: Cytoplasm or Nucleus
Positive control: Human ovarian cancer
Recommended dilution: 20-100
The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using ml263239(UPF3A Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using ml263239(UPF3A Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: ×200)