Product overview
Description Anti-GFAP rabbit polyclonal antibody
Applications ELISA, WB, IHC
Immunogen Synthetic peptide of human GFAP
Reactivity Human, Mouse, Rat
Content 0.8 mg/ml
Host species Rabbit
Ig class Immunogen-specific rabbit IgG
Purification Antigen affinity purification
Target information
Symbol GFAP
Full name glial fibrillary acidic protein
Synonyms ALXDRD
Swissprot P14136
Target Background
This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Applications
Immunohistochemistry
Predicted cell location: Cytoplasm
Positive control: Human liver cancer
Recommended dilution: 25-100 Predicted cell location: Cytoplasm
Positive control: Human esophagus cancer
Recommended dilution: 25-100
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using ml262817(GFAP Antibody) at dilution 1/35, on the right is treated with synthetic peptide. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using ml262817(GFAP Antibody) at dilution 1/35, on the right is treated with synthetic peptide. (Original magnification: ×200)
Western blotting
Predicted band size:50 kDa
Positive control:Mouse brain tissue lysate
Recommended dilution: 200-1000