Anti-VAMP2 antibody

Anti-VAMP2 antibody

Cat. No.     JC262474
Package    25 μl/100 μl/200 μl
Storage    -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Product overview
Description                   Anti-VAMP2 rabbit polyclonal antibody
Applications        ELISA, WB, IHC 
Immunogen               Synthetic peptide of human VAMP2
Reactivity                 Human, Mouse, Rat
Content                     0.3 mg/ml
Host species               Rabbit
Ig class                  Immunogen-specific rabbit IgG
Purification                 Antigen affinity purification

Target information
Symbol                 VAMP2
Full name                 vesicle-associated membrane protein 2 (synaptobrevin 2)
Synonyms    SYB2; VAMP-2
Swissprot    P63027

Target Background
The protein encoded by this gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. This gene is thought to participate in neurotransmitter release at a step between docking and fusion. The protein forms a stable complex with syntaxin, synaptosomal-associated protein, 25 kD, and synaptotagmin. It also forms a distinct complex with synaptophysin. It is a likely candidate gene for familial infantile myasthenia (FIMG) because of its map location and because it encodes a synaptic vesicle protein of the type that has been implicated in the pathogenesis of FIMG.

Applications
Immunohistochemistry
Predicted cell location: Cytoplasm
Positive control: Human thyroid cancer
Recommended dilution: 25-100    Predicted cell location: Cytoplasm
Positive control: Human esophagus cancer
Recommended dilution: 25-100
            
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using ml262474(VAMP2 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200)    The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using ml262474(VAMP2 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200)
Western blotting
Predicted band size:13 kDa
Positive control:A172, Jurkat and Hela cells, Mouse brain and Human fetal brain tissue 
Recommended dilution: 500-2000