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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20˚C
- 英文名:
Calpastatin
- 库存:
详询
- 供应商:
南京莱富赛
- 规格:
详询
host_species:E.coli
purification:>90% by SDS-PAGE
specificity:Human
other_names:Calpain inhibitor,Sperm BS-17 component
accession_no:P20810
Gene name:CAST
calculated_mw:
tag info:His
Immunogen Description:CDPMSSTYIEELGKREVTIPPKYRELLA
formulation:50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole,10%glycerol(PH8.0)
storage:Store at -20C. (Avoid repeated freezing and thawing.)
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
background:Specific inhibition of calpain (calcium-dependent cysteine protease). Plays a key role in postmortem tenderization of meat and have been proposed to be involved in muscle protein degradation in living tissue.
产品详细信息请点击:Calpastatin
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文献和实验Preparation of Calpastatin Samples for Western Blotting
Determination of calpastatin levels in cells or tissues by Western blotting using antibodies, or by assay of calpain inhibitory activities in vitro, may be required to assess the role and the level of calpastatin, with respect to the levels
Purification of Recombinant Calpastatin Expressed in Escherichia coli
Calpastatin is an endogenous inhibitor protein specific for calpain (1 ,2 ). Other calpain-inhibiting reagents such as EDTA, E-64, leupeptin, and calpain inhibitor I (N -acetyl-L-leucyl-L-leucyl-L-norleucinal), are frequently used
Purification of - and m-Calpain and Calpastatin from Animal Tissues
This chapter describes procedures for purification of μ-calpain, m-calpain, and calpastatin simultaneously from a single tissue sample. The procedures and reagents described apply equally well to purification
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