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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20˚C
- 英文名:
Recombinant Human Desmoglein-1(DSG1),partial
- 库存:
详询
- 供应商:
南京莱富赛
- 规格:
详询
host_species:in vitro E.coli expression system
purification:Greater than 90% as determined by SDS-PAGE.
other_names:Cadherin family member 4 Desmosomal glycoprotein 1
accession_no:Q02413
calculated_mw:75.4 kDa
tag info:N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged
Immunogen Description:Expression Region:50-548aa
Sequence Info:Extracellular Domain
formulation:Tris-based buffer50% glycerol
storage:The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃,-80℃. The shelf life of lyophilized form is 12 months at -20℃,-80℃.
Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
background:Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.
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文献和实验In Vitro Pathogenicity Assay for Anti-desmoglein Autoantibodies in Pemphigus
in blister formation: an in vitro dissociation assay using primary human epidermal keratinocytes to assess pathogenicity of anti-Dsg3 autoantibodies, and an alternative method whereby anti-Dsg3 and Dsg1 autoantibodies are injected into organ-cultured human
Recombinant Antigens in Viral Diagnosis
, and require the preparation and at least partial purification of large amounts of viral antigen. Furthermore, these assays will not differentiate between recent and past infections. Because antigen production is nearly always dependent on virus growth
Screening cDNA Libraries Using Partial Probes to Isolate Full-Length cDNAs from Vascular Cells
The purpose of screening cDNA libraries is to isolate a particular cDNA clone encoding a mRNA and by implication, a protein, of interest. The screening is based on identification of the desired clone among a large number of recombinant clones
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