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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20˚C
- 英文名:
Recombinant Human Adiponectin receptor protein 1(ADIPOR1)
- 库存:
详询
- 供应商:
南京莱富赛
- 规格:
详询
host_species:in vitro E.coli expression system
purification:Greater than 85% as determined by SDS-PAGE.
other_names:Progestin and adipoQ receptor family member I
accession_no:Q96A54
calculated_mw:62.6 kDa
tag info:N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged
Immunogen Description:Expression Region:1-375aa
Sequence Info:Full Length
formulation:Tris-based buffer50% glycerol
storage:The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃,-80℃. The shelf life of lyophilized form is 12 months at -20℃,-80℃.
Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
background:Receptor for ADIPOQ, an essential hormone secreted by adipocytes that regulates glucose and lipid metabolism. Required for normal glucose and fat homeostasis and for maintaining a normal body weight. ADIPOQ-binding activates a signaling cascade that leads to increased AMPK activity, and ultimately to increased fatty acid oxidation, increased glucose uptake and decreased gluconeogenesis. Has high affinity for globular adiponectin and low affinity for full-length adiponectin
产品详细信息请点击:Recombinant Human Adiponectin receptor protein 1(ADIPOR1)
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文献和实验In this chapter we describe efficient procedures for the construction, expression and screening of comprehensive libraries of human Fab antibody fragments displayed on the surface of filamentous phage. Phagemid vectors are used for placing
a procedure for the isolation of nondenatured, recombinant human AR with more than 95% purity using four-step chromatography with milligram yields (see Notes 1 -Notes 4 ). Purified AR may be used in physical and biochemical studies, such as monoclonal antibody
and p75) (2 ). Signaling through the p55 receptor is primarily associated with responses such as cytotoxicity (2 ,3 ) and cytokine secretion (4 ) whereas the p75 receptor is responsible for lymphoproliferative signals and the activation of T-cells
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