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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20˚C
- 英文名:
Recombinant Mouse Bloom syndrome protein homolog(Blm),partial
- 库存:
详询
- 供应商:
南京莱富赛
- 规格:
详询
host_species:E.coli
purification:Greater than 90% as determined by SDS-PAGE.
other_names:RecQ helicase homolog
accession_no:O88700
calculated_mw:24.7 kDa
tag info:N-terminal 10xHis-tagged and C-terminal MYC-tagged
Immunogen Description:Expression Region:684-859aa
Sequence Info:Partial
formulation:Tris-based buffer50% glycerol
storage:The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃,-80℃. The shelf life of lyophilized form is 12 months at -20℃,-80℃.
Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
background:Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction. Involved in 5'-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA. Negatively regulates sister chromatid exchange (SCE)
产品详细信息请点击:Recombinant Mouse Bloom syndrome protein homolog(Blm),partial
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文献和实验embryonic stem (ES) cells. Recessive genetic screens are feasible in ES cells that are Bloom-syndrome protein (Blm-) deficient. Therefore, we constructed a reporter cell line in Blm-deficient ES cells to isolate RNAi mutants through a simple drug-selection
depends on product and its function. N-terminal sequencing revealed that the purified rBoNTC(Hc )-N is missing the first eight amino acids of the N-terminus of the protein, whereas the purified rBoNTC(Hc )-C protein is intact. After a mouse bioassay test
Nucleic Acid Programmable Protein Arrays: Versatile Tools for Array‐Based Functional Protein Studies
Protein microarrays offer a global perspective on the function of expressed gene products. However, technical issues related to the stability and dynamic range of microarrays printed with purified protein have hampered their widespread adoption. Taking
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