Recombinant Mouse RNA polymera
se II subunit A C-terminal domain phosphatase(Ctdp1),partial- 询价
- Signalway Antibody
- USA
- AP77218
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Store at -20˚C
- 英文名：
Recombinant Mouse RNA polymerase II subunit A C-terminal domain phosphatase(Ctdp1),partial
- 库存：
详询
- 供应商：
南京莱富赛
- 规格：
详询
host_species:E.coli
purification:Greater than 90% as determined by SDS-PAGE.
other_names:TFIIF-associating CTD phosphatase
accession_no:Q7TSG2
calculated_mw:24 kDa
tag info:N-terminal 10xHis-tagged and C-terminal Myc-tagged
Immunogen Description:Expression Region:178-341aa
Sequence Info:Partial
formulation:Tris-based buffer50% glycerol
storage:The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃,-80℃. The shelf life of lyophilized form is 12 months at -20℃,-80℃.
Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
background:Processively dephosphorylates 'Ser-2' and 'Ser-5' of the heptad repeats YSPTSPS in the C-terminal domain of the largest RNA polymerase II subunit. This promotes the activity of RNA polymerase II. Plays a role in the exit from mitosis by dephosphorylating crucial mitotic substrates (USP44, CDC20 and WEE1) that are required for M-phase-promoting factor (MPF),CDK1 inactivation
产品详细信息请点击：Recombinant Mouse RNA polymerase II subunit A C-terminal domain phosphatase(Ctdp1),partial
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文献和实验Whole-mount in situ hybridization for the detection of RNA in C. elegans embryos
Geraldine Seydoux and Andrew Fire Adapted from Seydoux, G. and Fire, A. (1995). Whole-mount in situ hybridization for the detection of RNA in C. elegans embryos. In C. elegans: Modern Biological Analysis of an Organism. Methods
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
. Biol. In press, 1994. *Fields-Berry, S.C., A.L. Halliday, and C.L. Cepko. 1992. A recombinant retrovirus encoding alkaline phosphatase confirms clonal boundary assignment in lineage analysis of murine retina. Proc. Natl. Acad. Sci. USA. 89
96-well RNA In Situ Hybridization Protocol
digest can be used directly.2. Using a multichannel pipettor, add 5μlof 2X polymerase mix.3. Incubate at 37 degrees C for 2 hrs.4. Add 10μlDNase I mix.5. Incubate at 37 degrees C for 15 min.6. Using multichannel pipettor, add 80μl125 mM NaCO3 pH 10.2
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