purification:Greater than 90% as determined by SDS-PAGE.
other_names:Staphylolytic protease
accession_no:P14789
calculated_mw:36 kDa
tag info:N-terminal 6xHis-SUMO-tagged
Immunogen Description:Expression Region:237-418aa Sequence Info:Full Length of Mature Protein
formulation:Tris-based buffer50% glycerol
storage:The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃,-80℃. The shelf life of lyophilized form is 12 months at -20℃,-80℃. Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
background:Involved in proteolysis and elastolysis (degradation of the host protein elastin). Has staphylolytic activity (degrades pentaglycine cross-links in cell wall peptidogylcan), preferring Gly-Gly-|-X substrates where X is Ala or Gly (PubMed:11179971). Enhances the elastolytic but not proteolytic activity of elastase (lasB) and elastolytic activity of other proteases (PubMed:2110137). Degradation of host elastin is likely to contribute to the pathogenicity of P.aeruginosa. While either His-317 or His-356 can abstract a proton in the hydrolysis reaction, the same residue performs both functions in a given catalytic cycle, with the other stabilizing the catalytic intermediate