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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20˚C
- 英文名:
Recombinant Mouse ADP-ribosyl Cyclase,CD38 (C-6His)
- 库存:
详询
- 供应商:
南京莱富赛
- 规格:
详询
host_species:Human Cells
purification:Greater than 95% as determined by reducing SDS-PAGE.
specificity:Mouse
accession_no:P56528
calculated_mw:30.6kDa
formulation:Supplied as a 0.2 μm filtered solution of 20mM Tris,150mM NaCl,pH8.0.
reconstitution:
storage:Store at < -20C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.
产品详细信息请点击:Recombinant Mouse ADP-ribosyl Cyclase,CD38 (C-6His)
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文献和实验Purification of Recombinant Poly(ADP-Ribose) Polymerases
The purification of Poly(ADP-ribose) polymerases from overexpressing cells (Sf9 insect cells, Escherichia coli ) has been updated to a fast and reproducible three chromatographic steps protocol. After cell lysis, proteins from the crude
depends on product and its function. N-terminal sequencing revealed that the purified rBoNTC(Hc )-N is missing the first eight amino acids of the N-terminus of the protein, whereas the purified rBoNTC(Hc )-C protein is intact. After a mouse bioassay test
in a variety of signaling pathways. ADP-ribosylation not only uncouples these G-proteins from their receptors but in addition also deranges the adenylate cyclase system and in this way interferes with second-messenger pathways (3 –7 ). The modification
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