- 询价
- Signalway Antibody
- USA
- AP74853
- 2026年02月03日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Store at -20˚C
- 英文名：
Recombinant Human E3 Ubiquitin-Protein Ligase CHIP,CHIP
- 库存：
详询
- 供应商：
南京莱富赛
- 规格：
详询
host_species:E.coli
purification:Greater than 95% as determined by reducing SDS-PAGE.
specificity:Human
accession_no:Q9UNE7
calculated_mw:34.86kDa
formulation:Supplied as a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
reconstitution:Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg,ml.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
storage:Store at < -20C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.
产品详细信息请点击：Recombinant Human E3 Ubiquitin-Protein Ligase CHIP,CHIP
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文献和实验Reconstitution of CHIP E3 Ubiquitin Ligase Activity
CHIP, the carboxyl-terminus of Hsp70 interacting protein, is both an E3 ubiquitin ligase and an Hsp70 co-chaperone and is implicated in the degradation of cytosolic quality control and numerous disease substrates. CHIP has been shown
Purification and Characterization of Legionella U-Box-Type E3 Ubiquitin Ligase
LubX is one such bacterial E3 ubiquitin ligase. We describe here the method to purify soluble LubX protein using GST-tag and Escherichia coli overexpression systems. Using the purified protein together with recombinant ubiquitin, E1, and E2 enzymes
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
O. 45. Test the success of the ChIP reaction using 1 µL of undiluted immunoprecipitate by PCR with primers specific for a chromatin region known to be occupied by the protein of interest in the cell type under investigation. Cloning
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