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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 库存:
99
- 保质期:
已收到实际货物为准
- 供应商:
北京诺博莱德科技有限公司
- 保存条件:
已收到实际货物为准
详细信息:
| 克隆号 : | 1A4 |
| Ig分类 : | IgG1/K |
| 适用组织: | 冷冻切片/石蜡切片 |
| 阳性部位: | 细胞质 |
| 阳性对照: | 平滑肌 |
| 特异性 : | 此抗体可以与平滑肌的α机动蛋白反应,而与骨骼肌、心肌的肌动蛋白无交叉反应。可以识别人、狒狒、牛、兔、小鼠和鸡的相应抗原,其它种属没有检测。 |
| 推荐效价: | •原液使用前应以适当比例稀释 推荐稀释度为1:100~1:200 •工作液不必稀释,直接滴加使用 |
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文献和实验Western Blotting with Monoclonal Ant
the enzyme conjugate anti-mouse IgG:HRPO (Catalog# M15345) 1:2000 in wash buffer containing 5% non-fat dry milk (or 1% BSA for phosphotyrosine antibodies). 3.Decant the wash buffer, add the diluted enzyme conjugate and incubate with agitation for 30 minutes
Determination of Muscle Fiber Type in Rodents
of the hindlimb, were stained using rabbit polyclonal anti‐MYH7 (Sigma, cat. no. HPA001239) or mouse monoclonal BA‐D5. A typical checkerboard pattern is obtained with both antibodies. Dark unstained fibers are type IIa fibers, which do not express MyHC
Western Blotting with Monoclonal Antibodies
for 30 minutes with agitation, changing the wash buffer every 3? minutes. Dilute the enzyme conjugate anti-mouse IgG:HRPO (Catalog# M15345) 1:2000 in wash buffer containing 5% non-fat dry milk (or 1% BSA for phosphotyrosine antibodies).
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