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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
14-3-3 Theta Rabbit mAb
- 抗体英文名:
14-3-3 Theta Rabbit mAb
- 应用范围:
WB, ICC/IF, FC
- 宿主:
Recombinant Rabbit
- 适应物种:
Human;Mouse;Rat
- 抗原来源:
Recombinant Rabbit
- 供应商:
南京赛戈巍生物科技有限公司
- 保存条件:
Store at -20°C
- 免疫原:
recombinant protein
purification
ProA affinity purified
applications
WB, ICC/IF, FC
appl_detail
WB: 1:1,000-5,000
ICC: 1:100-1:500
FC: 1:50-1:100
other_names
14-3-3 antibody
14-3-3 protein T-cell antibody
14-3-3 protein tau antibody
14-3-3 protein theta antibody
1C5 antibody
HS1 antibody
theta polypeptide antibody
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein antibody
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta isoform antibody
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide antibody
calculated_mw
28 kDa
formulation
1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
uniprot
P27348
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文献和实验7.8 or Binding Buffer.(6) BD tubing 14-170-12F (Fisher).(7) Polyethylene tubing Clay Adanes.(8) 18 Gauge needles.(9) Binding Buffer (BioRad): make 1L. 314g/L ddH2O and filter through 0.22 um, filter. pH=9.0.(10) Elution buffer (BioRad): make 500 ml. 11g/500 ml
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
T-Cell Activation Using mAb to CD3
-0031 , or Purified, Cat. No. 14-0031 ) Complete RPMI-1640 Sterile single-cell suspension of mouse spleen or lymph nodes 96-well flat-bottom microtiter plates with lids (Costar Cat. No. 3596) MTT Buffer MTT Lysing Solution
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