PMS2 Rabbit mAb[48717]

别名:DNA mismatch repair gene homologue antibody
DNA mismatch repair protein PMS2 antibody
H_DJ0042M02.9 antibody
HNPCC4 antibody
Mismatch repair endonuclease PMS2 antibody
Mismatch repair gene PMSL2 antibody
PMS 2 antibody
PMS1 protein homolog 2 antibody
PMS2 antibody
PMS2 postmeiotic segregation increased 2 antibody
PMS2 postmeiotic segregation increased 2 (S. cerevisiae) antibody
PMS2_HUMAN antibody
PMS2CL antibody
PMSL2 antibody
Postmeiotic segregation increased, S. cerevisiae, 2 antibody

计算分子量:96 kDa
配方:1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
应用详情:WB: 1:1,000-1:2,000
IHC: 1:50-1:100
ICC: 1:50-1:200

FC: 1:50-1:100
图片:

Western blot analysis of PMS2 on Hela cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:1,000 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.

,

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-PMS2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ET1605-1) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

,

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-PMS2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ET1605-1) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

,

ICC staining PMS2 in Hela cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Carbonic anhydrase 2 monoclonal antibody at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS. Alexa Fluorc? 488 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

,

,

Flow cytometric analysis of PMS2 was done on Hela cells. The cells were fixed, permeabilized and stained with APE1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). After incubation of the primary antibody on room temperature for an hour, the cells was stained with a Alexa Fluor? 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.