- 询价
- Signalway Antibody
- 48140
- 2025年09月23日
- WB
- Rabbit
企业认证
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
peptide
- 形态：
liquid
- 保存条件：
Store at -20˚C
- 克隆性：
Polyclonal
- 保质期：
12 months
- 抗原来源：
Rabbit
- 供应商：
南京赛戈巍生物科技有限公司
- 宿主：
Rabbit
- 应用范围：
WB
- 抗体英文名：
6X His-Tag HRP Antibody
- 规格：
50ul/100ul
应用详情:WB: 1:2,000-1:5,000
图片:
Fig1 Western blot analysis of extracts from cells expressing C-terminal His-tagged protein 6X His-tag antibody: Lane1: 1:2,000 diluted antibody Lane2: 1:5,000 diluted antibody
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文献和实验Conjugation of Antibody to HRP
of Maleimide:HRP Weigh out 1.067 mg HRP for each mg of thiolated antibody. Add 1.1 mg Sulfo-SMCC per mg of HRP Add PBS to a final concentration of 8 mg/ml HRP Mix for 2 hours on a rotator. Desalt the HRP on PBS equilibrated column. Collect
Conjugation of Antibody to HRP
Conjugation of Antibody to HRP Author: Nanci Donacki Source: Contributed by Nanci Donacki Reagents
Monoclonal Antibody Production Protocol
- PBS + 0.1% Tween 20 or TBS + 0.1% Tween 20. - Blocking solution = 2% BSA (type V ) in PBS. (Add 0.02% azide for longer storage.) - Elisa buffer = 2% BSA + 0.1% Tween 20 in PBS ( azide optional ). - Enzyme linked antibody = Horseradish peroxidase
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